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GEOBIOLOGY 2002
AN INTERNATIONAL TRAINING COURSE
IN A RAPIDLY EVOLVING FIELD

Offered by the
USC Wrigley Institute for Environmental Studies
Sponsored by
The Agouron Institute

Open to students and researchers at the graduate,
postgraduate and professorial level

A DAY BY DAY SUMMARY

July 7th Symposium I ("The Chemistry Behind Life", Benner, Ellington, Yokobayashi, Switzer, Szostak)
July 8th Lectures (Benner:Introduction to Organic Chemistry; Ellington:What is Life?)

Laboratory (Spontaneous resolution of chirality)

Student Presentations
July 9th Lectures (Benner: Introduction to Organic Chemistry, II)

Laboratory (Liesegang Structures: Inorganic Stromatolites?)

Discussion (Szostak: What is Life?)
July 10th Lectures (Andy Ellington: Genetic Replicators, Steve Benner: Reconstructing the Historical Record of Life on Earth)

Laboratory (Belousov - Zhabotinskii Reaction)

Student Presentations
July 11th Lectures (Steve Benner: Bioinformatics, Andy Ellington: Have Genetic Replicators Left Fingerprints)

Laboratory (Belousov - Zhabotinskii Reaction II)

Student Presentations
July 12th Lectures (Andy Ellington: Boundary Conditions for Living Systems - How Can Life be Engineered?, Steve Benner: Bioinformatics II)

Laboratory (Introduction to GenBank and the Master Catalogue)

Student Presentations
July 13th Free Day
July 14th Depart for Field
Trip and Lectures
(Andy Knoll: Introduction to Paleoenvironments, John Grotzinger: Introduction to Mono Lake)
July 15th Lecture (Tim Hollibaugh: Biogeochemistry of Mono Lake)

Field (Mono Lake tufa, Hwy 395 ikaite, Hot Creek travertine)

Lectures (Andy Knoll: Geological Record at the Precambrian/Cambrian Boundary, Cambrian Explosion of Metazoan Life, John Grotzinger: Geological Record at the Precambrian/Cambrian Boundary II, Frank Corsetti: White-Inyo Section of the Precambrian/Cambrian Boundary)
July 16th Field (Poleta Folds Cambrian trace fossils, Mt. Morrisson Precambrian/Cambrian Boundary Cloudina)
July 17th Field (Crystal Spring Formation and Beck Springs dolomite stromatolites, Kingston Peak diamictite)

Lectures (John Grotzinger: Stromatolites as a Proxy Record of Life, Andy Knoll: The eukaryotic fossil record and phylogenetic tree, John Grotzinger: Snowball Earth)
July 18th Field (Noonday dolomite cap carbonate, tube structures, Johnnie Formation, Zabrieskie Quartzite trace fossils, Carrera Formation trilobites, Bonanza King Formation thrombolites, China Ranch Date Farm)

Discussion (John Grotzinger and Frank Corsetti: Snowball Earth, Noonday dolomite, and tubes)
July 19th Return from
Field Trip

July 20th Symposium II ("Stromatolites: Linking the Past and Present", Farmer, Canfield, Visscher, Golubic, Prufert-Bebout)
July 21st Sail Boat Trip
July 22nd Lectures (Jim Kasting: Origins of Life: How and When Did it Occur?, Brad Bebout: Biogeochemistry of Microbial Mat Ecosystems)

Laboratory (Introduction to Geochemical Methods, Introduction to Field Sites for the Week)
July 23rd Field Work (Sample Collection from Marine and Freshwater Field Sites)

Lectures (Jim Kasting: Long Term Climate Evolution, Unisense: New Methods for Biogeochemistry)

Laboratory (Geochemical and FISH Characterizations of Microbial Mats at the Field Sites)
July 24th Laboratory (Geochemical and FISH Characterizations of Microbial Mats at the Field Sites)

Lectures (Jim Kasting: Snowball Earth, David Des Marais: Biogeochemical Cycles of Carbon and Sulfur)

Lecture (Joe Kirschvink: Methane Fuse for Cambrian Explosion, Carbon Cycles, and True Polar Wander)
July 25th Lectures (Jim Kasting: Rise of Atmospheric Oxygen on Early Earth, Will Berelson: Measuring Gradients and Determining Oxygen Fluxes in Water/Sediment Column)

Laboratory (Geochemical and FISH Characterizations of Microbial Mats at the Field Sites)
July 26th Lectures (Jim Kasting: Liquid Water on Mars, David Des Marais: Biosignatures, Jim Kasting: Circumstellar Habitable Zones and the Search for Extraterrestrial Life)

Student Group
Presentations of Lab
and Field Work

July 27th Free Day (trip to Avalon)
July 28th Free Day
July 29th Lectures (Kurt Hanselmann: Unifying Concepts in Microbial Diversity and Evolution, Scott Dawson: So You Want to Know Some Microbial Ecology)

Laboratory (Luminous and Magnetotactic bacteria, Transposon Mutagenesis, FISH and Cloning of 16S rRNA Genes)

Discussion (Kurt Hanselmann: The Art of Designing Microbial Diets)
July 30th Lecture (Ken Nealson: Thermodynamics, Kinetics, and Geobiology: Are There Really Any Rules?, Dianne Newman: Geobiology: Co-Evolution of Life and Earth - How are Biofilms Relevant?)

Laboratory (Luminous and Magnetotactic bacteria, Transposon Mutagenesis, FISH and Cloning of 16S rRNA Genes)

Laboratory and
Demonstrations
(Solving Thermodynamic Problems with Thermodyn, Demo of Deep UV Microscope and Laser Raman Spectrometer)
July 31st Lectures (Ken Nealson: Iron and Manganese Oxidation in Nature and in the Laboratory, Dianne Newman: Bacterial Genetics: What It Has to Offer Geobiology I)

Laboratory (Luminous and Magnetotactic bacteria, Transposon Mutagenesis, FISH and Cloning of 16S rRNA Genes)

Laboratory and
Demonstrations
(Solving Thermodynamic Problems with Thermodyn, Demo of Deep UV Microscope and Laser Raman Spectrometer)
Aug 1st Lectures (Ken Nealson: Iron and Manganese Reduction in Nature and in the Laboratory, Dianne Newman: Bacterial Genetics: What It Has to Offer Geobiology II)

Discussion
and Lecture
(Radu Popa: Magnetotactic Bacteria, Biominerals, and a Putative Mineral Biosphere, Tanja Bosak: Growth of Biofilms, Scott Dawson: Environmental and Cell Biology Approaches in Eukaryote Evolution)
Aug 2nd Lectures (Kurt Hanselmann: Microbial Responses to Environmental Variability, Kurt Hanselmann: Evolution of Metabolic Pathways)

Student Group
Presentations of
Lab Work

Aug 3rd Symposium III ("Current Aspects of Microbes, Communities and Metals", Bassler, Kolter, Beveridge, Lovley, Banfield)
Aug 4th Free Day (Trip to the interior to see Arabian Horses)
Aug 5th Lectures (Gary Olsen: Introduction to Genomics I, Tom Schmidt: Genomic Analyses of Uncultivated Microbial Communities)

Laboratory (FISH, DNA extraction and PCR)
Aug 6th Lecture (Gary Olsen: Introduction to Genomics II, Tom Schmidt: Sequencing DNA)

Laboratory (FISH, DNA extraction and PCR, rRNA analysis and phylogeny )
Aug 7th Laboratory (FISH, Gel Electrophoresis and Cloning, Genome Analysis and Inference of Function)

Lecture (Gary Olsen: Functional Analysis of Genomes)
Aug 8th Lecture (Tom Schmidt: Phylogenomic analysis, Gary Olsen: Introduction to Phylogenetic Trees)

Laboratory (FISH, PCR and RFLP, rRNA analysis and phylogeny)
Aug 9th Lecture (Gary Olsen: Methods for inferring phylogenetic trees; Brad Stevenson (Cultivating the 'uncultivatable')

Laboratory (PCR and RFLP, Genome analysis and the inference of phylogeny)
Aug 10th Student Projects
Aug 11th Student Projects
Aug 12th Student Projects
Aug 13th Presentation of
Student Projects

Aug 14th Presentation of
Student Projects

Aug 15th Presentation of
Student Projects


Graduation

Farewell Party
Aug 16th Departure from
Catalina Island

TAKE A LOOK BACK AT THE 2002 COURSE

FOLLOW THE COURSE DAY BY DAY
MINISYMPOSIA TOPICS AND SUMMARIES
STUDENT PRESENTATION TOPICS
2002 COURSE DESCRIPTION

For further information:

Geobiology Course Committee
c/o Ann Close
Wrigley Institute for Environmental Studies
University of Southern California
3616 Trousdale Parkway
Los Angeles, California, USA 90089-0371
phone: 213-740-6780, fax: 213-740-6720
email: Ann Close, close@usc.edu